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random n6  (New England Biolabs)


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    Structured Review

    New England Biolabs random n6
    Random N6, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+n6+primers/Random+Primer+6/pmc12533556-229-26-28
    Average 94 stars, based on 442 article reviews
    random n6 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
    Article Snippet: .. RNA was quantified spectrophotometrically with Infinite 200 NanoQuant microplate reader (TECAN, Männedorf, Switzerland), and 1000 ng of RNA was used for cDNA synthesis. cDNA was generated with M-MLV RT reverse transcriptase (28025013, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) using random N6 primers (S1230S, NEB, Ipswich, MA, USA). .. Real-time qPCR was performed using FastStart Universal SYBR Green Master (Rox) (Roche, Merck, Darmstadt, Germany) on a 7500 fast real-time PCR system (Applied Biosystems, Waltham, MA, USA), with primers to detect the linear or circRNA transcripts ( ).

    Generated:

    Article Title: Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
    Article Snippet: .. RNA was quantified spectrophotometrically with Infinite 200 NanoQuant microplate reader (TECAN, Männedorf, Switzerland), and 1000 ng of RNA was used for cDNA synthesis. cDNA was generated with M-MLV RT reverse transcriptase (28025013, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) using random N6 primers (S1230S, NEB, Ipswich, MA, USA). .. Real-time qPCR was performed using FastStart Universal SYBR Green Master (Rox) (Roche, Merck, Darmstadt, Germany) on a 7500 fast real-time PCR system (Applied Biosystems, Waltham, MA, USA), with primers to detect the linear or circRNA transcripts ( ).

    Reverse Transcription:

    Article Title: Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
    Article Snippet: .. RNA was quantified spectrophotometrically with Infinite 200 NanoQuant microplate reader (TECAN, Männedorf, Switzerland), and 1000 ng of RNA was used for cDNA synthesis. cDNA was generated with M-MLV RT reverse transcriptase (28025013, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) using random N6 primers (S1230S, NEB, Ipswich, MA, USA). .. Real-time qPCR was performed using FastStart Universal SYBR Green Master (Rox) (Roche, Merck, Darmstadt, Germany) on a 7500 fast real-time PCR system (Applied Biosystems, Waltham, MA, USA), with primers to detect the linear or circRNA transcripts ( ).

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA.
    Article Snippet: Cell-free RNA was extracted from 3 ml of human plasma sing Quick-cfRNA Serum & Plasma Kit (Zymo Research, 1059) and treated with DNaseI (NEB, M0303) according to anufacturers’ protocols. .. Reverse transcription with induro RT and size selection for concatemeric circRNA cDNAs 4 μg of total RNA from human brain (BioChain, Catalog #: R1234035-P, Lot #: C404081), HEK293 cells or lung tumor (BioChain, Catalog #: R1235152-50, Lot #: A808151) or ribo-depleted and RNaseR treated RNA (500–700 ng) from human brain and HEK293 cells were incubated with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 10 μl at 72 ◦C for 5 minutes then cooled down to 4 ◦C with 0.1 ◦C / s ramp. .. ibodepletion and RNase R digestion of RNA 0 μg RNA from human brain (BioChain, Catalog #: 1234035-P, Lot #:C404081) and HEK293 cells were deleted of rRNA by NEBNext rRNA Depletion Kit v2 Human / Mouse / Rat) (NEB, E7400X) according to manuacturer’s protocol.

    Incubation:

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
    Article Snippet: Remaining RNAs were treated with 200 units of RNase R (Lucigen, RNR07250) at 37°C for 30 min, then purified with NEBNext RNA Sample Purification Beads (NEB, E6351). .. 4 μg of total RNA from human brain (BioChain, Catalog #: R1234035-P, Lot #: C404081), HEK293 cells or lung tumor (BioChain, Catalog #: R1235152-50, Lot #: A808151) or ribo-depleted and RNaseR treated RNA (500–700 ng) from human brain and HEK293 cells were incubated with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 10 μl at 72°C for 5 minutes then cooled down to 4°C with 0.1°C/s ramp. .. Then 2 μl 10mM dNTPs and 400 units of Induro RT (NEB, M0681) were added to a final volume of 40 μl in the 1× Induro-RT buffer supplemented with 3 mM MgCl 2 .

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA.
    Article Snippet: Cell-free RNA was extracted from 3 ml of human plasma sing Quick-cfRNA Serum & Plasma Kit (Zymo Research, 1059) and treated with DNaseI (NEB, M0303) according to anufacturers’ protocols. .. Reverse transcription with induro RT and size selection for concatemeric circRNA cDNAs 4 μg of total RNA from human brain (BioChain, Catalog #: R1234035-P, Lot #: C404081), HEK293 cells or lung tumor (BioChain, Catalog #: R1235152-50, Lot #: A808151) or ribo-depleted and RNaseR treated RNA (500–700 ng) from human brain and HEK293 cells were incubated with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 10 μl at 72 ◦C for 5 minutes then cooled down to 4 ◦C with 0.1 ◦C / s ramp. .. ibodepletion and RNase R digestion of RNA 0 μg RNA from human brain (BioChain, Catalog #: 1234035-P, Lot #:C404081) and HEK293 cells were deleted of rRNA by NEBNext rRNA Depletion Kit v2 Human / Mouse / Rat) (NEB, E7400X) according to manuacturer’s protocol.

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
    Article Snippet: .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C. .. Then 2 μl 10 mM dNTPs and 400 units of Induro RT (NEB, M0681) were added to a final volume of 40 μl in the 1× Induro-RT buffer supplemented with 3 mM MgCl 2 .

    Size Selection:

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA.
    Article Snippet: Cell-free RNA was extracted from 3 ml of human plasma sing Quick-cfRNA Serum & Plasma Kit (Zymo Research, 1059) and treated with DNaseI (NEB, M0303) according to anufacturers’ protocols. .. Reverse transcription with induro RT and size selection for concatemeric circRNA cDNAs 4 μg of total RNA from human brain (BioChain, Catalog #: R1234035-P, Lot #: C404081), HEK293 cells or lung tumor (BioChain, Catalog #: R1235152-50, Lot #: A808151) or ribo-depleted and RNaseR treated RNA (500–700 ng) from human brain and HEK293 cells were incubated with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 10 μl at 72 ◦C for 5 minutes then cooled down to 4 ◦C with 0.1 ◦C / s ramp. .. ibodepletion and RNase R digestion of RNA 0 μg RNA from human brain (BioChain, Catalog #: 1234035-P, Lot #:C404081) and HEK293 cells were deleted of rRNA by NEBNext rRNA Depletion Kit v2 Human / Mouse / Rat) (NEB, E7400X) according to manuacturer’s protocol.

    Clinical Proteomics:

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
    Article Snippet: .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C. .. Then 2 μl 10 mM dNTPs and 400 units of Induro RT (NEB, M0681) were added to a final volume of 40 μl in the 1× Induro-RT buffer supplemented with 3 mM MgCl 2 .

    other:

    Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA.
    Article Snippet: cDNAs were size selected twice using NEBNext DNA Sample Purification Beads (NEB, E6178) by incubating with 0.97% PEG8000 and 97 mM MgCl 2 for 30 minutes ( 26 ).



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